PCR Supplies
Boca Scientific Inc Maxime PCR PreMix Kit (i-Taq), 480 Tubes
Maxime PCR PreMix Kit (i-Taq™) contains all components necessary for a PCR reaction in one tube in a dried, stable format. PCR can be carried out immediately with the addition of the template, primer and distilled water, reducing the risk of pipetting error. Gel loading electrophoresis is also possible as the tubes contain loading dye. This kit features Intron’s i-Taq™ DNA Polymerase, a highly purified (anion exchange) polymerase of 94 kDa. The buffer composition has been optimized to obtain the best polymerase activity regardless of the template type or reaction conditions. This kit has excellent stability due to oxidation and moisture prevention achieved by a vacuum compression manufacturing method. Each tube contains i-Taq™ DNA Polymerase (5U/ul), dNTPs, reaction buffer (10x), and gel loading buffer.
Boca Scientific Inc SD Polymerase, High Concentration, 50 U/ul, 1000 U
SD Polymerase is a novel, artificial polymerase with a strong strand displacement activity and heat stability up to 93°C. With SD Polymerase, the possibilities of isothermal amplification and regular PCR can be combined. The high heat stability of SD Polymerase enhances the efficiency of isothermal amplification by initial denaturation steps to increase the accessibility of single strands. The initial heating-up improves the specificity in applications such as LAMP, library generation, PCDR, WGA, standard PCR, and genome sequencing. The special properties of SD Polymerase provide greater yields, speed and efficiency than Taq Polymerase. SD Polymerase is suitable for high GC-content and difficult targets. Long Range PCR can be performed with target sizes up to 30 kb. SD Polymerase has 5’-3’ polymerase and 5’-3’ strand displacement activities, as well as 3’-A-overhangs. It does not have any exonuclease activity.
LAMDA BIOTECH INC Taq Plus 2x MasterMixno color
A pre-formulated PCR master mix containing all components necessary for standard PCR amplification. Available in two three color variants for direct gel loading capability.
Alkali Scientific Radiant 2X HotStart Taq Mastermix, 40 Reactions,(1 x 1mL)
Radiant™ 2X Hot-Start Taq Mastermix Description RadiantTM 2x Hot-Start Taq Mastermix is a ready-to-use, robust PCR mix containing our highly purified Hot-Start Taq DNA Polymerase, reaction buffer, ultra-pure dNTPs, MgCl2 and PCR enhancers. RadiantTM 2x Hot-Start Taq Mastermix is optimized for the sensitive DNA amplification of a wide range of DNA templates including complex mammalian genomic DNA and crude samples. The Hot-Start technology is based on a new-generation PCR buffer formulation in conjunction with a proprietary antibody-mediated chemistry. RadiantTM Hot-Start Taq Mastermix exhibits 5´-3´ DNA polymerase activity with an error-rate of wild-type Taq ( 2.0 x 10-5). The polymerase mix is ideal for genotyping, colony PCR, multiplexing, low-copy assays, and the amplification of challenging targets susceptible to mispriming. RadiantTM Hot Start Taq Mastermix is engineered for robust, superior PCR and is supplied as a ready-to-use 2x mix which provides exceptional sensiti
Alkali Scientific Radiant™ qPCR Genotyping Mix, No-ROX, Suitable for Dual-labeled Taqman™ and Other Probes, 100 Reactions, (1 X 1mL)
Using the latest advancements in polymerase technology and advanced buffer chemistry Radiant™ Genotyping Mix No-Rox offers market leading performance with minimal optimization. Radiant™ Genotyping Mix No-Rox is a kit designed for use in dual-labeled probe based genotyping assays including Taqman, Molecular Beacon and Scorpion probe genotyping. Radiant™ GenotypingMix is fully compatible with ABI TaqMan Pre-Designed SNP Genotyping assays. Radiant™ Genotyping Mix uses proprietary small molecular inhibitor technology that prevents formation of primer-dimers to improve reaction sensitivity and specificity. Highthroughput screening has resulted in a buffer system that allows efficient amplification from GC-rich and AT-rich templates, under fast and standard cycling conditions.
Alkali Scientific ProCycle Fast Optical qPCR Plates, Half-Skirted, 96 x 0.1ml Well, H12 notch, 10/Pk
ProCycle Fast Optical qPCR Plates, HalfSkirted, 96 x 0.1mL Well, H12 notch, 10/pk
LUNA NANOTECH INC Nanoelute Gel Extraction Kit, 50 Preps
PuroSPIN™ NanoElute™ Gel Extraction Kit, 50 Preps
The PuroSPIN™ NanoElute™ Gel Extraction Kit is designed to provide a simple and easy-to-use method for extraction and purification of DNA samples (100 bp – 10 kb) excised from agarose or polyacrylamide (PAGE) gels. This kit uses a special binding buffer formula that includes a pH indicator as a visual representation of optimal DNA binding conditions to the silica spin column.
In comparison to our standard PuroSPIN™ Gel Extraction Kit, the NanoElute version of the kit is supplemented with Nano silica spin columns instead of mini silica columns. Nano columns provide the highest possible concentration of purified DNA fragments as the elution volume needed is as little as 6 μL. Due to the high binding capacity of the column the kit can be used to purify high amounts of starting DNA sample (up to 10 ug), however, comparing to the standard kit as little as 50 ng of DNA can be succ
LUNA NANOTECH INC 0.2 ML Pcr Tube Magnetic Rack
- Fit individual 0.2 mL PCR tubes and 0.2 mL PCR tubes 8-strips
- 12 positions for 0.2 mL PCR tubes
- Housing material: PMMA/Acrylic
- Magnet: N38 Neodymium (NdFeB)"
LUNA NANOTECH INC Plasmid Miniprep Plus Kit, 96-Well, 384 Preps
PuroSPIN-96™ Plasmid Miniprep Plus Kit, 96-Well Plate Format, 384 Preps
The PuroSPIN-96™ Plasmid Miniprep Kit is designed to provide a simple and easy-to-use method for large-scale extraction and purification of plasmid DNA.
This purification technology is based on plasmid DNA binding to 96-well silica membrane purification plates, which allows for efficient removal of genomic DNA, protein, salt, and other contaminants. Silica purification plates provide a faster, simpler, and safer alternative to traditional nucleic acid purification techniques such as phenol-chloroform extraction.
In comparison to our standard PuroSPIN-96™ Plasmid Miniprep Kit, the Plus kit adds an additional filtration step that removes the precipitated SDS-protein complexes, genomic DNA, carbohydrates and other contaminants from the lysate before loading it onto the silica spin plates. This prevents contaminating materials from binding to and blocking parts of t
LUNA NANOTECH INC 6-Position Midi Magnetic Separation Rack For 15 ML Tubes
MIDI Magnetic Separation Rack for 15 mL Tubes
MIDI format magnetic racks for medium scale separation of magnetic beads in 15 mL centrifuge tubes. These racks can be used with our PuroMAG MIDI kits as well as various magnetic beads offered by Luna Nanotech.
Medium 15 mL tube volume makes the rack optimal when performing mdium scale magnetic separation reactions.
- Fits 15 mL centrifuge tubes.
- Available in 8-position format.
LUNA NANOTECH INC Ngsprep Size Selection Magnetic Beads, 500 ML
PuroMAG™ NGSPrep Magnetic Beads, 500 mL
The PuroMAG™ NGSPrep Magnetic Beads are designed for fast and highly effective DNA clean-up after polymerase chain reaction (PCR, qPCR, ddPCR), microarrays, sequencing and other types of ezymatic reactions by removing unincorporated primers, dNTPs (deoxynucleotide triphosphates), salts, and other impurities. Combination of SPRI technology and paramagnetic feature of the beads allows for specific size selection of DNA fragments during the clean-up procedure with high recovery of the DNA product. This is crucial application in library preparation for various sequencing techniques, including next-generation sequencing (NGS).
Features
- Easy and fast clean-up of DNA PCR samples with efficient recovery of the product
- Highly efficient size selection of purified fragmented genome or PCR product for NGS library prep
- High throughput clean-up of 96/384 samples
- Can be used
LUNA NANOTECH INC Viral Dna/Rna Purification Kit, Magnetic, 96 Preps
PuroMAG™ Viral DNA and RNA Purification Kit, Magnetic, 96 Preps
The PuroMAG™ Viral DNA and RNA Purification Kit is designed to provide a simple and easy-to-use method for extraction of viral genomic nucleic acids from blood, plasma, serum, buccal or nasal swabs, and saliva. This kit can be used for both DNA and RNA viruses as our buffer formulations and column membranes are specially optimized to bind both DNA and RNA nucleic acids. Our kit greatly simplifies the extraction process by incorporating a convenient and easy-to-use extraction technology based on nucleic acid binding to silica-coated magnetic beads.
High quality DNA and RNA extracted with the PuroMAG™ Viral DNA and RNA Purification Kit can be used for a range of downstream applications, such as reverse transcription or qPCR.
This kit offers both low and high throughput applications using a manual magnetic rack or automated purification systems. The high-throughput protoc
LUNA NANOTECH INC Total Rna Purification Kit, 250 Preps
PuroSPIN™ Total RNA Purification Kit, 250 Preps
PuroSPIN™ Total RNA Purification Kitis designed to purify total RNA from bacteria, mammalian cells, yeast, or animal tissue. It greatly simplifies the extraction process by incorporating a convenient easy-to-use extraction technology based on nucleic acid binding silica spin-columns. The RNA is first released form the cells by the treatment with the Lysis Buffer LB-R. For RNA extraction from bacterial cell the lysis step is preceded by the treatment with the Lysozyme enzyme to disrupt bacterial cell wall. RNA is then loaded onto silica-based spin columns and washed to remove protein, salt and other contaminants. The kit includes on-column DNase I digestion step to remove any contaminating DNA. Finally, purified RNA is eluted from the column into a clean microcentrifuge tube in Elution Buffer EB, or nuclease-free water.
The kit can also be used to perform RNA cleanup after DNase I or other enzymatic treatm